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Oxidative stress during leukocyte absorption apheresis
Author(s) -
Hirayama Aki,
Nagase Sohji,
Ueda Atsushi,
Ishizu Takashi,
Taru Yoshinori,
Yoh Keigyou,
Hirayama Kouichi,
Kobayashi Masaki,
Koyama Akio
Publication year - 2003
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.10054
Subject(s) - tbars , oxidative stress , apheresis , medicine , antioxidant , absorption (acoustics) , thiobarbituric acid , chromatography , immunology , pharmacology , chemistry , biochemistry , lipid peroxidation , platelet , materials science , composite material
Leukocyte absorption apheresis absorbs leukocytes to the apheresis columns involving leukocyte activation. This process is regarded as bioincompatible and avoided in hemodialysis or other extracorporeal circulation processes. Thus, leukocyte apheresis has a potential risk to exacerbate in vivo oxidative stress. We evaluated the changes in plasma oxidative stress during leukocyte apheresis. Patients diagnosed as ulcerative colitis (UC) and treated with leukocyte apheresis were studied. Adacolumn (celluloseacetate beads) or Cellsorba EX (polyethylenephtarate fiber) was used for the leukocyte absorption device. Oxidative stress was measured by thiobarbituric acid reactive substances (TBARS) and hydroxyl radical ( • OH) scavenging activity. Plasma samples were collected from the pre‐ and post‐column sampling port at the start, and from the pre‐column sampling port at the end of the treatment. The • OH signal intensities (OHRI) significantly increased during a column passage, indicating a loss of plasma • OH scavenging activity. However, OHRI was reduced at the end, suggesting a recovery of radical scavenging activity during leukocyte apheresis. Significant decreases of OHRI and TBARS were only observed in the early phase of the therapeutic course. No differences of OHRI and TBARS levels were observed between the two columns. These results indicate that though the plasma antioxidant activity was diminished by a column passage, plasma antioxidant activity recovers during the procedure. This efficient antioxidative effect is limited to the early phase of the therapeutic course. J. Clin. Apheresis 18:61–66, 2003. © 2003 Wiley‐Liss, Inc.