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Modification of the leukapheresis procedure for use in rhesus monkeys ( Macaca mulata )
Author(s) -
Ageyama Naohide,
Kimikawa Masaaki,
Eguchi Kei,
Ono Fumiko,
Shibata Hiroaki,
Yoshikawa Yasuhiro,
Terao Keiji
Publication year - 2003
Publication title -
journal of clinical apheresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.697
H-Index - 46
eISSN - 1098-1101
pISSN - 0733-2459
DOI - 10.1002/jca.10043
Subject(s) - leukapheresis , medicine , apheresis , hematocrit , peripheral blood mononuclear cell , plasmapheresis , immunology , andrology , surgery , platelet , stem cell , cd34 , biology , biochemistry , genetics , antibody , in vitro
One of the most serious problems in applying leukapheresis to human infants is the large extracorporeal blood volume (ECV), resulting in substantial loss of platelets and red blood cells (RBCs). In this study, we developed a safe and effective modified procedure to collect peripheral blood stem cells (PBSCs) from rhesus monkeys ( Macaca mulata ) using a Baxter CS3000+ Blood Cell Separator (Baxter, Deerfield, IL) with several devices that reduced chamber size and shortened the standard apheresis kit to decrease ECV from 130 to 70 ml. Pump speed was controlled by monitoring hematocrit values and platelet counts during leukapheresis. This system makes it possible to perform safe and effective leukapheresis in rhesus monkeys whose body weight is similar to that of human infants. A total of 12 leukapheresis procedures were performed in nine monkeys and resulted in the collection of sufficient numbers of white blood cells (mean, 1.38 × 10 9 cells/kg), CD34 + cells (mean, 17.80 × 10 6 cells/kg), mononuclear cells (mean, 3.67 × 10 8 cells/kg), and colony forming units (mean, 75.02 × 10 6 cells/kg) in all cases. In addition, no complications, such as anemia or trombocytopenia, occurred after leukapheresis. This modified leukapheresis procedure will be useful to test new approaches in gene therapy, perform organ transplantation using nonhuman primates, and collect PBSCs from human infants in a noninvasive manner. Our nonhuman primate model provides an important framework for such future clinical studies. J. Clin. Apheresis 18:26–31, 2003. © 2003 Wiley‐Liss, Inc.