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Increased oxidative stress and mitochondrial impairments associated with increased expression of TNF‐α and caspase‐3 in palmitic acid‐induced lipotoxicity in myoblasts
Author(s) -
Mansuri Mohammad Lukman,
Sharma Garima,
Parihar Priyanka,
Dube Kirti Tiwari,
Sharma Tejasweta,
Parihar Arti,
Parihar Mordhwaj Singh
Publication year - 2021
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22744
Subject(s) - lipotoxicity , oxidative stress , myocyte , palmitic acid , endocrinology , medicine , apoptosis , chemistry , reactive oxygen species , skeletal muscle , saturated fatty acid , fatty acid , biology , microbiology and biotechnology , biochemistry , insulin resistance , insulin
Saturated fatty acids, whose circulating levels are markedly increased in the body, significantly affect the growth and functions of skeletal muscle. These fatty acids may exert a detrimental effect on the undifferentiated skeletal myoblasts that may adversely affect their differentiation. In the present study, the exposure of myoblasts to excess palmitic acid caused an elevation of tumor necrosis factor‐α expression and an increase in reactive oxygen species levels consistent with the enhanced inflammation and oxidative stress. Various concentrations of palmitic acid significantly decreased the mitochondrial membrane potential, induced the programmed cell death by an increase in the caspase‐3 expression, and DNA fragmentation in the myoblasts. These findings suggest that the increased concentrations of saturated fatty acid in the myoblasts increase lipotoxicity by increasing inflammation and oxidative stress, decreasing the mitochondrial function, and inducing apoptosis.