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Coaction of hepatic thioredoxin and glutathione systems in iron overload‐induced oxidative stress
Author(s) -
Sönmez Aydın Feyza,
Hukkamlı Berna,
Budak Harun
Publication year - 2021
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22704
Subject(s) - thioredoxin , glutathione , oxidative stress , lipid peroxidation , thioredoxin reductase , glutathione reductase , malondialdehyde , hepcidin , ferritin , chemistry , gpx4 , glutathione peroxidase , ferroportin , apoptosis , biochemistry , microbiology and biotechnology , biology , superoxide dismutase , immunology , enzyme , inflammation
In the present study, we demonstrate the coaction of thioredoxin and glutathione (GSH) systems in mouse liver against iron overload‐induced oxidative stress (OS). Mice were injected intraperitoneally with an iron dextran solution twice a week for 3 weeks. Iron accumulation in mouse liver was demonstrated spectroscopically. To confirm the iron overload model in the liver, the increased gene expression levels of hepcidin ( Hamp ), ferroportin ( Fpn1 ), and ferritin ( Fth1 ), which regulate iron trafficking, were observed by a quantitative polymerase chain reaction. In the case of iron overload, the GSH level and the reduced glutathione/oxidized glutathione ratio, which represents a marker of OS, decreased significantly. An increase in the malondialdehyde level, one of the final products of the lipid peroxidation process, was observed. The gene expression of the thioredoxin system, including thioredoxin ( Trx1 ) and thioredoxin reductase ( TrxR1 ), was examined. Though TrxR1 expression decreased, no changes were observed in Trx1 . The enzyme activity and semiquantitative protein expression of TRXR1 increased. The activity of GSH reductase and GSH peroxidase increased in the iron overload group. The gene and protein expressions of thioredoxininteracting protein, which is an indicator of the commitment of the cell to apoptosis, were elevated significantly. The increased protein expression of Bcl‐2‐related X protein and CASPASE‐3, which is an indicator of apoptosis, increased significantly. In conclusion, excess iron accumulation in mouse liver tissue causes OS, which affects the redox state of the thioredoxin and GSH systems, inducing cell apoptosis and also ferroptosis due to increased lipid peroxidation and the depletion of GSH level.