Premium
Antiproliferative effect and autophagy induction of curcumin derivative ZYX02‐Na on the human lung cancer cells A549
Author(s) -
Zhou GuangZhou,
Guo ShuangShuang,
Liu DengXu,
Zhang Lu,
Sun GangChun
Publication year - 2020
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22592
Subject(s) - curcumin , a549 cell , acridine orange , chemistry , autophagy , flow cytometry , pi3k/akt/mtor pathway , viability assay , cell cycle , cell cycle checkpoint , microbiology and biotechnology , cell growth , cell , apoptosis , biochemistry , biology
At present, a large number of curcumin derivatives had been produced and identified aiming to replace the curcumin in view of its low bioavailability and stability. Here, a novel curcumin derivative ZYX02‐Na was first used to reduce the cell viability of human non–small cell lung cells A549, which was confirmed by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay. Flow cytometry and Western blot analysis showed that ZYX02‐Na could lead to cell cycle arrest in G0/G1 phase, which demonstrated that ZYX02‐Na inhibited the proliferation of A549 cells. Furthermore, the AMPK/mTOR/4E‐BP1 signaling pathway was activated in ZYX02‐Na‐treated A549 cells. Besides, wounding healing and transwell experiments showed that ZYX02‐Na could also inhibited the migration ability of A549 cells. Moreover, we also found that ZYX02‐Na could induce autophagy of A549 cells by acridine orange staining, GFP‐LC3 subcellular localization observation and Western blotting analysis, respectively. In short, our current studies indicated that ZYX02‐Na possessed the antiproliferation effect and autophagy induction on A549 cells, while in vivo anticancer study of ZYX02‐Na needs to be done in future.