Premium
Curcumin inhibits alloxan‐induced pancreatic islet cell damage via antioxidation and antiapoptosis
Author(s) -
Xia Zhenhong,
Jiang Xue,
Li Ke,
Li Lixia,
Chen Wenbo,
Wang Yuxiang,
Liu Yanqiang
Publication year - 2020
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22499
Subject(s) - curcumin , superoxide dismutase , islet , oxidative stress , reactive oxygen species , alloxan , chemistry , apoptosis , cell damage , malondialdehyde , microbiology and biotechnology , in vitro , pharmacology , biochemistry , biology , insulin , endocrinology , diabetes mellitus
The present study elucidates the possible protective effects of curcumin on β‐cells damaged by oxidative stress and its significance in controlling diabetes mellitus in in vitro experiments. Pancreatic islet (RIN‐m5F) cells were treated with 25 mmol/L alloxan (AXN) to induce cell damage and the protective effects of curcumin were observed. The results showed that curcumin significantly promoted the cellular activity of AXN‐treated RIN‐m5F cells, decreased the ratio of apoptosis, downregulated the level of malondialdehyde, upregulated the levels of superoxide dismutase and reactive oxygen species, increased the expression of Bcl‐2, cleaved caspase‐3, and cleaved PARP1, and decreased the expression of Bax in AXN‐treated cells. These results suggest that curcumin inhibits AXN‐induced damage in RIN‐m5F cells via antioxidative and antiapoptotic mechanisms.