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Bilobalide assuages morphine‐induced addiction in hippocampal neuron cells through upregulation of microRNA‐101
Author(s) -
Zhang Yan,
Zhai Hongyin
Publication year - 2020
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22493
Subject(s) - mapk/erk pathway , chemistry , morphine , pharmacology , western blot , downregulation and upregulation , microbiology and biotechnology , kinase , biology , biochemistry , gene
Bilobalide exhibits many biological activities, but its effects on morphine stimulation have not been elucidated. The research aims to explore the function and underlying mechanisms of bilobalide in morphine‐led hippocampal neuron cells. Cells were treated with or without morphine or oxaliplatin (OXA), bilobalide, or SCH772984 dilutions. miR‐101 inhibitor and negative control were transfected into cells. Western blot and quantitative reverse transcription‐polymerase chain reaction were, respectively, conducted to measure the relative expression of proteins or RNAs. Morphine improved the expression levels of orexin1 receptor (OX1R) and c‐FOS, the p/t‐ERK/PKC as well. The c‐FOS protein level and p/t‐ERK/PKC were significantly elevated by morphine + OXA. Bilobalide had no effect on OX1R and p/t‐PKC but evidently decreased the c‐FOS and p/t‐ERK. The p‐ERK and the c‐FOS accumulation levels were remarkably reduced by SCH772984. The production of miR‐101 was promoted by bilobalide but inhibited by the miR‐101 inhibitor. miR‐101 inhibitor abolished bilobalide's inhibitory effects on p/t‐ERK. Bilobalide exhibited morphine‐induced effects on hippocampal neuron cells by upregulating miR‐101.