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Effect of citronellol on NF‐kB inflammatory signaling molecules in chemical carcinogen‐induced mammary cancer in the rat model
Author(s) -
Jayaganesh Rajendran,
Pugalendhi Pachaiappan,
Murali Raju
Publication year - 2020
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22441
Subject(s) - dmba , carcinogen , carcinogenesis , inflammation , nitric oxide synthase , chemistry , cancer research , tumor necrosis factor alpha , mammary tumor , cancer , biology , medicine , immunology , biochemistry , breast cancer , enzyme
Inflammation plays a vital role in the process of carcinogenesis and anti‐inflammatory properties of phytochemicals are gaining more attention in the chemoprevention of cancer. The present study was designed to evaluate the anti‐inflammatory potential of citronellol (CT) on 7,12‐dimethylbenz( a )anthracene (DMBA)‐induced mammary carcinogenesis in rats. The inflammation‐associated gene and protein markers were analyzed by immunohistochemistry, reverse transcription polymerase chain reaction, and Western blot techniques. Markers such as nuclear factor‐kB (NF‐kB), tumor necrosis factor‐α, interleukin‐6 (IL‐6), cyclooxygenase‐2, macrophage inflammatory protein‐1α, and inducible nitric oxide synthase are upregulated in DMBA‐alone–treated mammary tumor tissues. The oral administration of CT (50 mg/kg BW) to DMBA‐treated rats significantly downregulated the expression NF‐kB and other inflammatory markers, and also increased the level of IL‐10 in mammary tissues. The results suggested that the anti‐inflammatory potential of CT prevented the incidence of chemical carcinogen‐induced mammary cancer in rats.