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Phenylethyl isothiocyanate induces oxidative damage of porcine kidney cells mediated by reactive oxygen species
Author(s) -
Zhu Yuanyuan,
Liu Shuiping,
Yan Sisi,
Wang Ji,
Zhang Linyu,
LI Xin,
Wen Lixin,
Wu Jing
Publication year - 2020
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22428
Subject(s) - reactive oxygen species , chemistry , oxidative phosphorylation , oxidative damage , oxidative stress , isothiocyanate , oxygen , kidney , biochemistry , microbiology and biotechnology , biology , endocrinology , organic chemistry
The aim of this study is to confirm the toxic effect of phenylethyl isothiocyanate (PEITC) on porcine kidney cells (PK‐15) and explore the effect of oxidative damage mediated by reactive oxygen species (ROS) induced by PEITC in PK‐15 cells. Porcine kidney cell line (PK‐15) was treated with PEITC (2, 5, and 10 µM) for 24 hours, and the oxidative damage mediated by PEITC through ROS was investigated. The survival rate of PK‐15 cells decreased in a dose‐dependent manner after the treatment of PEITC in a dose‐dependent manner. A high concentration of PEITC (10 µM) can change cell morphology, increase the content of malondialdehyde, ROS, and lactate dehydrogenase, and decrease the activity of SOD, CAT, GSH‐PX, and GSH. PEITC has a toxic effect on PK‐15 cells by inducing oxidative stress in PK‐15 cells through the generation of ROS.