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Raloxifene alleviates amyloid‐β‐induced cytotoxicity in HT22 neuronal cells via inhibiting oligomeric and fibrillar species formation
Author(s) -
Liu Ziyi,
Wang Youqiao,
Qin Wenjing,
Chen Daoyuan,
Feng Yanqiao,
Su Hui,
Shao Weiyan,
Zhou Binhua,
Bu Xianzhang
Publication year - 2019
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22395
Subject(s) - raloxifene , neuroprotection , selective estrogen receptor modulator , microglia , chemistry , cytotoxicity , pathogenesis , estrogen , amyloid (mycology) , estrogen receptor , pharmacology , toxicity , microbiology and biotechnology , cancer research , endocrinology , medicine , biochemistry , biology , inflammation , in vitro , cancer , inorganic chemistry , organic chemistry , breast cancer
Raloxifene, a selective estrogen receptor modulator, displays benefits for Alzheimer's disease (AD) prevention in postmenopausal women as hormonal changes during menopause have the potential to influence AD pathogenesis, but the underlying mechanism of its neuroprotection is not entirely clear. In this study, the effects of raloxifene on amyloid‐β (Aβ) amyloidogenesis were evaluated. The results demonstrated that raloxifene inhibits Aβ 42 aggregation and destabilizes preformed Aβ 42 fibrils through directly interacting with the N‐terminus and middle domains of Aβ 42 peptides. Consequently, raloxifene not only reduces direct toxicity of Aβ 42 in HT22 neuronal cells, but also suppresses expressions of tumor necrosis factor‐α and transforming growth factor‐β induced by Aβ 42 peptides, and then alleviates microglia‐mediated indirect toxicity of Aβ 42 to HT22 neuronal cells. Our results suggested an alternative possible explanation for the neuroprotective activity of raloxifene in AD prevention.