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Prenatal exposure to bisphenol‐A altered miRNA‐224 and protein expression of aromatase in ovarian granulosa cells concomitant with elevated serum estradiol levels in F 1 adult offspring
Author(s) -
Lite Christy,
Ahmed Sheik S. S. J.,
Santosh Winkins,
Seetharaman Barathi
Publication year - 2019
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22317
Subject(s) - endocrinology , medicine , aromatase , offspring , microrna , biology , steroidogenic acute regulatory protein , andrology , pregnancy , gene expression , gene , biochemistry , genetics , cancer , breast cancer
This study was aimed to predict bisphenol‐A (BPA)‐responsive miRNA's using an in silico approach and to study their expression in granulosa cells of animals exposed prenatally to BPA. Pregnant Wistar rats were exposed to BPA through water (25 μg/L, 250 μg/L, and 2.5 mg/L) during gestation. The expression of miRNA‐133b, miRNA‐378 and miRNA‐224 were analyzed in ovarian granulosa cells. BPA affected the postnatal developmental landmarks such as weight of the pups at birth and reduced anogenital distance. BPA exposed animals showed elevated serum estradiol (E2) levels, while follicle‐stimulating hormone levels were reduced. The expression of miRNA‐224 and aromatase protein levels were found to be increased. This preliminary finding reveals the impact of early life exposure to BPA on the long‐term ovarian functions that may be mediated through miRNA‐based granulosa cell response. Besides, it is also a compelling indicator for the subclinical response that could have important consequences on female fertility.