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Subchronic intravenous toxicity study of biofunctional ZnO and its application as a fluorescence probe for cell‐specific targeting
Author(s) -
Nakamura Morihiko,
Watanabe Natsuko
Publication year - 2019
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22276
Subject(s) - in vivo , biocompatibility , chemistry , toxicity , conjugated system , cell , preclinical imaging , nanoparticle , antibody , antigen , biophysics , macrophage , nanotechnology , in vitro , cancer research , materials science , medicine , biochemistry , immunology , biology , polymer , microbiology and biotechnology , organic chemistry
Successful development of safe and highly effective nanoprobes for targeted imaging of in vivo early cancer is a great challenge. Herein, we choose the visible‐light emitting zinc oxide non–core/shell type nanoparticle (NP) fluorophores (ZHIE) as prototypical materials. We have reported on these materials previously. The results showed that the ZHIE NPs exhibited good water solubility and good biocompatibility. This study was conducted to investigate the toxicity of ZHIE NPs when intravenously administered to mice repeatedly at the dose required for successful tumor imaging in vivo. Anti‐macrophage‐1 antigen (Mac1), a macrophage differentiation antigen, antibody‐conjugated ZHIE NPs successfully realized targeted imaging of murine macrophage cell line Raw264.7 cells. In conclusion, ZHIE NPs are not toxic in vivo and antibody‐conjugated ZHIE NPs have great potential in applications, such as single cell labeling.