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Preventive effect of apigenin against isoproterenol‐induced apoptosis in cardiomyoblasts
Author(s) -
Thangaiyan Radhiga,
Robert Beaulah Mary,
Arjunan Sundaresan,
Govindasamy Kanimozhi,
Nagarajan Rajendra Prasad
Publication year - 2018
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22213
Subject(s) - apigenin , apoptosis , chemistry , cytochrome c , dna damage , oxidative stress , pharmacology , reactive oxygen species , flavonoid , biochemistry , microbiology and biotechnology , dna , biology , antioxidant
We investigated the effect of apigenin, a dietary flavonoid, on isoproterenol hydrochloride (ISO)‐induced apoptotic signaling in cardiomyoblast H9C2 cells. The results showed that apigenin treatment (10 µM) prevented ISO (31.25 μM)‐induced lipid peroxidative levels and antioxidants status in H9C2 cells. Furthermore, apigenin inhibited expression of inflammatory markers in ISO‐treated cells. In addition, apigenin prevented ISO‐induced DNA damage and apoptotic signaling through modulating the expression of Bax, caspase‐3, ‐8 and ‐9, cytochrome c, and Fas proteins in H9C2 cells. It is concluded that apigenin prevents ISO‐induced antioxidants depletion, oxidative DNA damage, inflammatory, and apoptotic signaling in H9C2 cells. Thus, the present results demonstrated that apigenin has a cardioprotective effect on cardiomyoblasts cells.