z-logo
Premium
[6]‐Gingerol–induced cell cycle arrest, reactive oxygen species generation, and disruption of mitochondrial membrane potential are associated with apoptosis in human gastric cancer (AGS) cells
Author(s) -
Mansingh Debjani P.,
O. J. Sunanda,
Sali Veeresh Kumar,
Vasanthi Hannah R.
Publication year - 2018
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22206
Subject(s) - reactive oxygen species , apoptosis , acridine orange , cancer cell , chemistry , mitochondrion , gingerol , ethidium bromide , inner mitochondrial membrane , annexin , membrane potential , biochemistry , microbiology and biotechnology , biology , cancer , dna , chromatography , genetics
Ginger ( Zingiber officinale Roscoe ), a monocotyledonous herb, is widely used as an herbal medicine owing to the phytoconstituents it possesses. In the current study, the quantity of [6]‐gingerol, the major phenolic ketone, in the fresh ginger and dried ginger rhizome was found to be 6.11 µg/mg and 0.407 µg/mg. Furthermore, [6]‐gingerol was assessed for its antiapoptotic effects in human gastric adenocarcinoma (AGS) cells evidenced by acridine orange/ethidium bromide staining technique and Annexin‐V assay. An increase in reactive oxygen species (ROS) generation led to a decrease in mitochondrial membrane potential (MMP) and subsequent induction of apoptosis. Results disclose that perturbations in MMP are associated with deregulation of Bax/Bcl‐2 ratio at protein level, which leads to upregulation of cytochrome‐c triggering the caspase cascade. These enduringly suggest that [6]‐gingerol can be effectively used for targeting the mitochondrial energy metabolism to manage gastric cancer cells.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here