Premium
Inhibition effects of pesticides on glutathione‐ S ‐transferase enzyme activity of Van Lake fish liver
Author(s) -
Özaslan Muhammet Serhat,
Demir Yeliz,
Aksoy Mine,
Küfrevioğlu Ömer Irfan,
Beydemir Şükrü
Publication year - 2018
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22196
Subject(s) - isopropylamine , cyhalothrin , chemistry , dichlorvos , pesticide , glutathione , glutathione s transferase , biochemistry , carbaryl , enzyme assay , chromatography , enzyme , biology , agronomy
Glutathione‐ S ‐transferases (GSTs) have a function in xenobiotic metabolism. They are a significant multifunctional family with a wide variety of catalytic activities. In the current study, we determined in vitro inhibition effects of 2,4‐dichlorophenoxyacetic acid dimethylamine salt (2,4‐D DMA), haloxyfop‐ P ‐methyl, glyphosate isopropylamine, dichlorvos, and λ‐cyhalothrin on purified GST. For this purpose, GST were purified from Van Lake fish (Chalcalburnus tarichii Pallas ) liver with 29.25 EU mg −1 specific activity and 10.76% yield using GSH–agarose affinity chromatographic method. The pesticides were tested at various concentrations on in vitro GST activity. K i constants were calculated as 0.17 ± 0.01, 0.25 ± 0.05, 3.72 ± 0.32, 0.42 ± 0.06, and 0.025 ± 0.004 mM, for 2,4‐D DMA, haloxyfop‐ P ‐methyl, glyphosate isopropylamine, dichlorvos, and λ‐cyhalothrin, respectively. λ‐Cyhalothrin showed a better inhibitory effect compared to the other pesticides. The inhibition mechanisms of λ‐cyhalothrin were competitive, while the other pesticides were noncompetitive.