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Evaluation of the toxicity effects of silk fibroin on human lymphocytes and monocytes
Author(s) -
Naserzadeh Parvaneh,
Mortazavi Seyed Alireza,
Ashtari Khadijeh,
Salimi Ahmad,
Farokhi Mehdi,
Pourahmad Jalal
Publication year - 2018
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22056
Subject(s) - toxicity , oxidative stress , chemistry , reactive oxygen species , apoptosis , fibroin , cytochrome c , mitochondrion , microbiology and biotechnology , biochemistry , mitochondrial toxicity , adenosine triphosphate , silk , biology , organic chemistry , computer science , operating system
Silk fibroin nanoparticles (SFNPs) as a natural polymer have been utilized in biomedical applications such as suture, tissue engineering‐based scaffolds, and drug delivery carriers. Since there is little data regarding the toxicity effects on different cells and tissues, we aimed to determine the toxicity mechanisms of SFNPs on human lymphocytes and monocytes based on reliable methods. Our results showed that SFNPs (0.5, 1, and 2 mg/mL) induced oxidative stress via increasing reactive oxygen species production, mitochondrial membrane potential (∆Ψ) collapse, which was correlated to cytochrome c release and Adenosine diphosphate (ADP)/Adenosine tri phosphate (ATP) ratio increase as well as lysosomal as another toxicity mechanism, which led to cytosolic release of lysosomal digestive proteases, phosphor lipases, and apoptosis signaling. Taken together, these data suggested that SFNPs toxicity was associated with mutual mitochondrial/lysosomal cross‐talk and oxidative stress on human lymphocytes and monocytes with activated apoptosis signaling.