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Co‐exposure to fluoride and sulfur dioxide on histological alteration and DNA damage in rat brain
Author(s) -
Wang Chong,
Liang Chen,
Ma Junjie,
Manthari Ram Kumar,
Niu Ruiyan,
Wang Jinming,
Wang Jundong,
Zhang Jianhai
Publication year - 2018
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22023
Subject(s) - neurotoxicity , chemistry , sulfur dioxide , comet assay , fluoride , dna damage , staining , toxicity , sodium fluoride , sulfur , toxicology , microbiology and biotechnology , biochemistry , pharmacology , environmental chemistry , dna , pathology , biology , medicine , inorganic chemistry , organic chemistry
Fluoride (F) and sulfur dioxide (SO 2 ) are the two common environmental contaminants that are associated with neurotoxicity. The present study was conducted to explore individual and combined exposure effects of F and SO 2 on histological alteration and DNA damage in rat brain. For this, male Wistar albino rats were exposed to sodium fluoride (100 mg/L NaF) and sulfur dioxide (39.3 mg/m 3 ) individually and in combination for 8 weeks. Histological alteration in brain is evaluated by hematoxylin–eosin staining, showed shrunken neurons, darkly stained small nucleus and decreased cell numbers in F and SO 2 exposed groups. The effect of F and SO 2 on DNA damage was assessed by comet assay. The results showed an increase in ratio of tailing and tail length in F or/and SO 2 administered rats. In addition, the proportion of grade II and III were also increased in individual and combined exposed groups. Compared with the individual exposure, the proportion the grade III was significantly high in combined exposure, suggesting a synergistic effect of F and SO 2 . These results indicate that the brain was more susceptible to the toxic effects of F and SO 2 . And combined exposure to these pollutants can lead more pronounced toxic effects on brain.