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Potential cow milk xanthine oxidase inhibitory and antioxidant activity of selected phenolic acid derivatives
Author(s) -
Nile Shivraj Hariram,
Keum Young Soo,
Nile Arti Shivraj,
Kwon Young Deuk,
Kim Doo Hwan
Publication year - 2018
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22005
Subject(s) - chemistry , xanthine oxidase , abts , dpph , antioxidant , food science , cow milk , uncompetitive inhibitor , non competitive inhibition , biochemistry , enzyme
Xanthine oxidase (XO) found in all mammals and excess activity leads to urolithiasis. The cow milk XO was purified to 305‐fold with a specific activity of 8.76 EU/mg and overall yield of 87% by using DEAE‐Sepharose chromatography. The phenolics showed potent XO inhibitory effect with K i , P1 (0.412), P2 (0.632), P3 (0.585), P4 (0.886), P5 (1.633), P6 (0.503), P7 (2.882), P8 (3.761), P9 (4.487), and P10 (5.841) μM. The phenolics P9 and P10 exhibited uncompetitive inhibition; the phenolics P1, P2, P3, P4, and P6 showed competitive inhibition, and other phenolic acids showed noncompetitive inhibition. The studied phenolic compounds showed potent antioxidant activity and expressed as EC 50 , ranged from, DPPH (4.2–25.8 μg mL –1 ), ABTS (10.2–42.5 mmol TE 100 g –1 ), and FRAP (6.3–36.8 mol Fe (II) 100 g –1 ). The results obtained from this study might be utilized for design of XO inhibitors and as antigout agent.