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In vitro and in vivo antithrombotic and cytotoxicity effects of ferulic acid
Author(s) -
Choi JunHui,
Park JongKook,
Kim KiMan,
Lee HyoJeong,
Kim Seung
Publication year - 2018
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22004
Subject(s) - ferulic acid , in vivo , antithrombotic , chemistry , platelet , pharmacology , cytotoxicity , in vitro , biochemistry , thrombin , platelet activation , immunology , medicine , biology , microbiology and biotechnology
We discovered recently in vitro and in vivo antithrombotic and cytotoxicity effects of ferulic acid. The cytotoxicity assays showed that ferulic acid (∼300 μg/mL) did not cause any significant toxicity on three cell lines, platelets, leukocytes, and erythrocytes. In vitro assays showed inhibitory effects of ferulic acid on thrombin (THR)‐ or collagen/epinephrine‐stimulated platelet activation by inhibiting platelet aggregation, and decreasing clot retraction activity. The in vitro effect of ferulic acid on THR‐stimulated platelet activation was proved by the decrease in the secretion of serotonin from the platelets. The anticoagulant effects of ferulic acid were confirmed by the prolongation of the intrinsic or/and extrinsic pathways and the delay of recalcification time in plasma coagulation. Ferulic acid had antithrombotic effect in acute thromboembolism model in vivo , and decreased the expression of α IIb β 3 /FIB and phosphorylation of AKT in THR‐stimulated platelet activation in vivo , and their antithrombotic efficacies hold promise for therapeutic targeting in our ongoing studies.