Premium
Triggering p53 activation is essential in ziyuglycoside I‐induced human retinoblastoma WERI‐Rb‐1 cell apoptosis
Author(s) -
Zhu Xue,
Wang Ke,
Yao Yong,
Zhang Kai,
Zhou Fanfan,
Zhu Ling
Publication year - 2018
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.22001
Subject(s) - downregulation and upregulation , retinoblastoma , apoptosis , chemistry , viability assay , cancer research , retinoblastoma protein , cancer cell , cell cycle , cancer , biology , biochemistry , gene , genetics
Ziyuglycoside I (Ziyu I), one of the major components isolated from the root of Sanguisorba officinalis L., has been proved for the antitumor properties on oral cancer, prostate cancer, and colorectal cancer. However, the effect of Ziyu I on retinoblastoma (RB) is not well understood. In this study, we investigated the inhibitory effect and underlying molecular mechanism of Ziyu I on human RB WERI‐Rb‐1 cells. Our results indicated that Ziyu I could suppress cell viability and induce mitochondrial‐dependent cell apoptosis in WERI‐Rb‐1 cells. Furthermore, Ziyu I treatment increased p53 expression as well as improved p53 stabilization through downregulation of pS166‐Mdm2 and upregulation of phosphorylated‐ and acetylated‐p53. Blockade of p53 significantly attenuated Ziyu I‐induced mitochondrial dysfunction. Our findings demonstrate that Ziyu I exhibits excellent anticancer effect on human RB WERI‐Rb‐1 cells by triggering p53 activation, and imply Ziyu I as a potential compound for chemotherapy of human RB.