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Attenuation of DNA damage and mRNA gene expression in hypoxic rats using natural antioxidants
Author(s) -
Fadda Laila Mohamed,
Attia Hala A.,
AlRasheed Nouf Mohamed,
Ali Hanaa Mahmoud,
Aldossari Manal
Publication year - 2017
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21975
Subject(s) - carnosine , dna damage , oxidative stress , sodium nitrite , downregulation and upregulation , chemistry , apoptosis , gene expression , reactive oxygen species , hypoxia (environmental) , microbiology and biotechnology , tumor necrosis factor alpha , pharmacology , endocrinology , dna , gene , biology , biochemistry , oxygen , organic chemistry
This study aimed to explore the efficiency of carnosine (Cs) and/or l ‐arginine (Agn) in the downregulation of apoptotic and inflammatory molecule expression and DNA damage caused hepatic injury in response to sodium nitrite (Sd)‐induced hypoxia in rats. Rats were injected with Sd; Agn or/and Cs were administrated prior to Sd intoxication. Sd significantly decreased hemoglobin concentration and Bcl‐2 mRNA expression, while increased expressions of apoptotic markers (Bax and caspase), tumor necrosis factor‐α, nuclear factor kappa B, and C‐reactive protein and the oxidative DNA damage in hepatic tissue. Moreover, administration of Agn or/and Cs exhibited a modulation of the previous parameters. However, concurrent treatment with the forementioned antioxidants modulated these levels. It was concluded that the treatment with the combination of Agn and Cs was the most effective regimen in ameliorating Sd toxicity accompanied by hypoxic stress.