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Sirtuin7 is involved in protecting neurons against oxygen‐glucose deprivation and reoxygenation‐induced injury through regulation of the p53 signaling pathway
Author(s) -
Lv Jianrui,
Tian Junbin,
Zheng Guoxi,
Zhao Jing
Publication year - 2017
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21955
Subject(s) - gene knockdown , apoptosis , ischemia , reperfusion injury , microbiology and biotechnology , chemistry , biology , medicine , biochemistry
Sirtuin7 (SIRT7) is known to regulate apoptosis and stress responses. So far, very little is known about the role of SIRT7 in cerebral ischemia/reperfusion injury. In this study, we aimed to investigate the potential role of SIRT7 in regulating oxygen‐glucose deprivation and reoxygenation (OGD/R)‐induced injury in neurons. We found a significant increase of SIRT7 expression in neurons in response to OGD/R treatment. Knockdown of SIRT7 aggravated OGD/R‐induced injury. Knockdown of SIRT7 augmented the levels of total and acetylated p53 protein. Moreover, knockdown of SIRT7 markedly increased the transcriptional activity of p53 toward apoptosis and activated the p53‐mediated proapoptotic signaling pathway. By contrast, overexpression of SIRT7 showed the opposite effects. Taken together, the results of our study suggest that SIRT7 is involved in protecting neurons against OGD/R‐induced injury, possibly through regulation of the p53‐mediated proapoptotic signaling pathway, indicating a potential therapeutic target for cerebral ischemia/reperfusion injury.