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Effect of astaxanthin and aluminum chloride on erythrocyte G6PD and 6PGD enzyme activities in vivo and on erythrocyte G6PD in vitro in rats
Author(s) -
Temel Yusuf,
Bengü Aydın Şükrü,
Akkoyun Hurrem Turan,
Akkoyun Mahire,
Ciftci Mehmet
Publication year - 2017
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21954
Subject(s) - in vivo , enzyme , glucose 6 phosphate dehydrogenase , chemistry , enzyme assay , in vitro , lactate dehydrogenase , biochemistry , dehydrogenase , microbiology and biotechnology , biology
In this study, we investigated the effect of astaxanthin (Ast) and aluminum (Al) on the erythrocyte glucose‐6‐phosphate dehydrogenase (G6PD) and 6‐phosphogluconate dehydrogenase (6PGD) enzymes activities in vivo and on G6PD enzyme in vitro in rats. For in vitro studies, G6PD enzyme was purified from rat erythrocyte by using 2′,5′‐ADP‐Sepharose 4B affinity gel. The effects of Ast and Al 3+ ion were investigated on the purified enzyme. It was determined that Ast increased the enzyme activity, whereas Al 3+ inhibited the enzyme activity noncompetitively (IC 50 values; 0.679 mM, K i values 1.32 mM). For in vivo studies, the rats were divided into the groups: control (Cont.), Al, Ast, and Al + Ast. The last three groups were compared with the control group. In Al group, a significant degree of inhibition was observed in the activity of G6PD and 6PGD enzymes when compared with the control group ( P < 0.05), whereas there was an increase in the activities of G6PD and 6PGD enzymes in Ast and Al + Ast groups ( P < 0.05).