Premium
The neuroprotective effect of mesenchymal stem cells on an experimentally induced model for multiple sclerosis in mice
Author(s) -
Mahfouz Marwa M.,
Abdelsalam Rania M.,
Masoud Marwa A.,
Mansour Hanaa A.,
AhmedFarid Omar A.,
kenawy Sanaa A.
Publication year - 2017
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21936
Subject(s) - experimental autoimmune encephalomyelitis , mesenchymal stem cell , multiple sclerosis , neuroprotection , medicine , encephalomyelitis , immunology , intraperitoneal injection , tumor necrosis factor alpha , central nervous system , pharmacology , methylprednisolone , nitric oxide , pathology
Abstract Multiple sclerosis (MS) is a chronic autoimmune demyelinating neurodegenerative central nervous system disorder. The aim of the present study was to investigate the prophylactic effect exerted by the one‐time intraperitoneal injection of mesenchymal stem cells (MSCs) 1 × 10 6 and 14‐day intraperitoneal injection of methylprednisolone (MP) 40 mg/kg in an experimental autoimmune encephalomyelitis (EAE). EAE was induced by intradermal injection of rat spinal cord homogenate with complete Freund's adjuvant in Swiss mice. Results of MSCs and MP‐treated mice showed a significantly milder disease and fewer clinical scores compared to control mice. They suppressed tumor necrosis factor‐alpha and myeloperoxidase and increased interleukin 10, whereas thiobarbituric acid reactive substances and nitric oxide brain contents were reduced to comparable levels between treatment groups. Brain content of GSH was significantly higher in MSCs‐treated mice than control mice. It is evident that MSCs have relevant prophylactic effect in an animal model of MS and might represent a valuable tool for stem cell based therapy in MS.