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Antioxidant activities of novel resveratrol analogs in breast cancer
Author(s) -
Chatterjee Anwesha,
Ronghe Amruta,
Padhye Subhash B.,
Spade David A.,
Bhat Nimee K.,
Bhat Hari K.
Publication year - 2018
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21925
Subject(s) - reactive oxygen species , oxidative stress , resveratrol , antioxidant , superoxide dismutase , chemistry , dna damage , deoxyguanosine , breast cancer , transcription factor , cancer research , pharmacology , biochemistry , cancer , biology , dna , medicine , gene
The objective of the present study was to characterize the role of novel resveratrol (Res) analogs: 4‐(E)‐{(4‐hydroxyphenylimino)‐methylbenzene, 1, 2‐diol} (HPIMBD) and 4‐(E)‐{(p‐tolylimino)‐methylbenzene‐1,2‐diol} (TIMBD) as potent antioxidants against breast cancer. Non‐neoplastic breast epithelial cell lines MCF‐10A and MCF‐10F were treated with 17β‐estradiol (E2), Res, HPIMBD, and TIMBD for up to 72 h. mRNA and protein levels of antioxidant genes, superoxide dismutase 3 (SOD3) and N‐quinoneoxidoreductase‐1 (NQO1) and transcription factors, nuclear factor erythroid 2‐related factor (Nrf) 1, 2 and 3 were quantified after the above treatments. Generation of reactive oxygen species (ROS) was measured by CM‐H2‐DCFDA and oxidative‐DNA damage was determined by measuring 8‐hydroxy‐2‐deoxyguanosine (8‐OHdG). HPIMBD and TIMBD scavenged cellular ROS production, attenuated oxidative DNA damage, increased mRNA and protein expression levels of SOD3 and NQO1 and activated Nrf signaling pathway. Our studies demonstrate that HPIMBD and TIMBD have the potential as novel antioxidants to prevent development of breast cancer.