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Levels of heavy metals and their binding protein metallothionein in type 2 diabetics with kidney disease
Author(s) -
Raudenska Martina,
Dvorakova Veronika,
Pacal Lukas,
Chalasova Katarina,
Kratochvilova Monika,
Gumulec Jaromir,
RuttkayNedecky Branislav,
Zitka Ondrej,
Kankova Katerina,
Adam Vojtech,
Masarik Michal
Publication year - 2017
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21891
Subject(s) - metallothionein , oxidative stress , glutathione , antioxidant , chemistry , medicine , bilirubin , endocrinology , diabetes mellitus , type 2 diabetes mellitus , biochemistry , type 2 diabetes , enzyme , gene
Abstract Hyperglycemia, a major metabolic disturbance present in diabetes, promotes oxidative stress. Activation of antioxidant defense is an important mechanism to prevent cell damage. Levels of heavy metals and their binding proteins can contribute to oxidative stress. Antiradical capacity and levels of metallothionein (MT), metals (zinc and copper), and selected antioxidants (bilirubin, cysteine, and glutathione) were determined in 70 type 2 diabetes mellitus (T2DM) subjects and 80 healthy subjects of Caucasian origin. Single nucleotide polymorphism (rs28366003) in MT gene was detected. Antiradical capacity, conjugated bilirubin, and copper were significantly increased in diabetics, whereas MT and glutathione were decreased. Genotype AA of rs28366003 was associated with higher zinc levels in the diabetic group. The studied parameters were not influenced by renal function. This is the first study comprehensively investigating differences in MT and metals relevant to oxidative stress in T2DM. Ascertained differences indicate increased oxidative stress in T2DM accompanied by abnormalities in non‐enzymatic antioxidant defense systems.