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Isoflavone genistein inhibits estrogen‐induced chloride and bicarbonate secretory mechanisms in the uterus in rats
Author(s) -
Chinigarzadeh Asma,
Karim Kamarulzaman,
Muniandy Sekaran,
Salleh Naguib
Publication year - 2017
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21878
Subject(s) - genistein , endocrinology , uterus , medicine , phytoestrogens , chemistry , ovariectomized rat , cystic fibrosis transmembrane conductance regulator , estrogen receptor , diethylstilbestrol , estrogen , cotransporter , biology , cystic fibrosis , cancer , breast cancer , organic chemistry , sodium
We hypothesized that genistein could affect the chloride (Cl − ) and bicarbonate (HCO 3 − ) secretory mechanisms in uterus. Ovariectomized female rats were given estradiol or estradiol plus progesterone with 25, 50, or 100 mg/kg/day genistein. Following completion of the treatment, uterine fluid Cl − and HCO 3 − concentrations were determined by in vivo uterine perfusion. Uteri were subjected for molecular biological analysis (Western blot, qPCR, and immunohistochemistry) to detect levels of expression of Cystic Fibrosis transmembrane regulator (CFTR), Cl − /HCO 3 − exchanger (SLC26a6), Na + /HCO 3 − cotransporter (SLC4a4), and estrogen receptor (ER)‐α and β. Coadministration of genistein resulted in decrease in Cl − and HCO 3 − concentrations and expression of CFTR, SLC26a6, SLC4a4, and ER‐α and ER‐β in the uteri of estradiol‐treated rats. In estradiol plus progesterone‐treated rats, a significant increase in the above parameters were observed following high‐dose genistein treatment except for the SLC24a4 level. In conclusion, genistein‐induced changes in the uterus could affect the reproductive processes that might result in infertility.