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Hyperargininemia and renal oxidative stress: Prevention by antioxidants and N G ‐nitro‐ l ‐arginine methyl ester
Author(s) -
Delwingde Lima Daniela,
DelwingDal Magro Débora,
Vieira Cindy Laís Pett,
Grola Gislaine Maria Marestoni,
Fischer Débora Adriana,
Wyse Angela Terezinha
Publication year - 2017
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21830
Subject(s) - chemistry , oxidative stress , superoxide dismutase , glutathione peroxidase , ascorbic acid , endocrinology , catalase , arginine , nitric oxide , medicine , thiobarbituric acid , in vivo , antioxidant , biochemistry , glutathione , nitric oxide synthase , renal cortex , kidney , enzyme , lipid peroxidation , amino acid , biology , food science , microbiology and biotechnology , organic chemistry
We investigated the in vitro and in vivo effects of arginine (Arg) on thiobarbituric acid‐reactive substances (TBA‐RS) and on the activities of catalase (CAT), glutathione peroxidase (GSH‐Px), and superoxide dismutase (SOD) in renal tissues of rats. We also studied the influence of antioxidants (α‐tocopherol plus ascorbic acid) and nitric oxide synthase inhibitor N G ‐nitro‐ l ‐arginine methyl ester ( l ‐NAME) on the effects elicited by Arg. Results showed that Arg in vitro (1.5 mM) decreased SOD activity and increased the levels of TBA‐RS in the renal medulla. Acute administration of Arg [0.8 g/kg, intraperitoneal injection] decreased CAT activity, increased SOD activity and TBA‐RS levels in the renal medulla, and decreased CAT activity in the renal cortex of rats. Most results were prevented by antioxidants and/or l ‐NAME. Data indicate that Arg causes an oxidative imbalance in the renal tissues studied; however, in the presence of antioxidants and l ‐NAME, some of these alterations in oxidative stress were prevented.