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Estimation of the Oxidative Stress and Molecular Damage Caused by 1‐Butyl‐3‐Methylimidazolium Bromide Ionic Liquid in Zebrafish Livers
Author(s) -
Dong Miao,
Liu Tong,
Wang Jinhua,
Wang Jun,
Zhu Lusheng,
Zhang Jun
Publication year - 2016
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21782
Subject(s) - oxidative stress , malondialdehyde , zebrafish , superoxide dismutase , chemistry , bromide , reactive oxygen species , ionic liquid , dna damage , oxidative phosphorylation , oxidative damage , catalase , biochemistry , nuclear chemistry , medicinal chemistry , dna , microbiology and biotechnology , organic chemistry , biology , gene , catalysis
The present study investigated the toxic effects of 1‐butyl‐3‐methylimidazolium bromide ([C 4 mim]Br) in zebrafish livers after exposure to 5–40 mg/L of [C 4 mim]Br on days 7, 14, 21, and 28. The results showed that low levels of [C 4 mim]Br exposure activated the superoxide dismutase (SOD) activity during early periods of exposure. However, high concentrations (20–40 mg/L) of [C 4 mim]Br caused the inhibition of SOD, which, accordingly , led to excess reactive oxygen species and malondialdehyde. The present results indicate that [C 4 mim]Br causes oxidative stress in the livers of both male and female zebrafish. Additionally, the DNA damage revealed that [C 4 mim]Br has a genotoxic effect on zebrafish livers, even when the concentration of [C 4 mim]Br is low (5 mg/L). The DNA damage was demonstrated by quantifying the DNA strand breakage.