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Polydatin Induces Apoptosis and Inhibits Growth of Acute Monocytic Leukemia Cells
Author(s) -
Wang Chunmei,
Luo Yuan,
Lu Jie,
Wang Yingchao,
Sheng Guangyao
Publication year - 2016
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21779
Subject(s) - apoptosis , cell cycle , cyclin d1 , thp1 cell line , flow cytometry , chemistry , monocytic leukemia , acute monocytic leukemia , cyclin b1 , cell cycle checkpoint , cancer research , cell growth , cyclin a , leukemia , microbiology and biotechnology , cell culture , cyclin dependent kinase 1 , biology , immunology , biochemistry , genetics
Polydatin (PD), a component isolated from Polygonum cuspidatum , has various activities such as inhibiting platelet aggregation, lowering level of blood lipid, reducing lipid peroxidation, and so on. However, the antitumor activity of PD has been poorly reported. In the present study, effect of PD on cell proliferation was evaluated by Cell Counting Kit‐8, and cell cycle and apoptosis were investigated by flow cytometry. Meanwhile, the protein expression level of Bc1‐2, Bax, cyclin A, cyclin B, and cyclin D1, which associated with apoptosis and cell cycle were analyzed by Western blotting. Results show that PD could effectively inhibit the growth, arrest cells in S phase, and induce apoptosis of acute monocytic leukemia cell line THP‐1; meanwhile, expression of cyclin D1 and Bc1‐2 decreased significantly, and expression of Bax and cyclin A increased notably. All results suggest that PD maybe a potential therapeutic strategy for acute monocytic leukemia.