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Toxicity of Tributyltin in Juvenile Common Carp ( Cyprinus Carpio ): Physiological Responses, Hepatic Gene Expression, and Stress Protein Profiling
Author(s) -
Li ZhiHua,
Zhong LiQiao,
Mu WeiNa,
Wu YanHua
Publication year - 2016
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21767
Subject(s) - tributyltin , common carp , cyprinus , oxidative stress , carp , chemistry , toxicity , biology , biochemistry , fishery , environmental chemistry , fish <actinopterygii> , organic chemistry
In this study, the effects of tributyltin (TBT) on biochemical parameters (antioxidant responses and Na + ‐K + ‐ATPase) in different tissues were investigated by using juvenile common carp ( Cyprinus Carpio ) as well as growth and ion regulation–related genes expression and stress‐related proteins profiling in fish liver. Oxidative stress indices and Na + ‐K + ‐ATPase showed tissues‐specific responses in fish exposed to different TBT concentrations. All tested genes related to GH/IGF‐I axis and ion‐regulation were significantly induced in the TBT group with lower concentrations (except for the igfbp3 in 10 μg/L) and were inhibited in 20 μg/L. In addition, the profiling of two proteins Hsp 70 and MT were increasing in a dose‐dependent manner under TBT stress. In short, TBT‐induced biochemical and molecular responses in different tissues were reflected in the measured parameters in the test. On the basis of TBT residue levels in the natural environment, more long‐term experiments at lower concentrations will be necessary in the future.