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Antithrombotic Activities of Luteolin In Vitro and In Vivo
Author(s) -
Choi JunHui,
Kim YoonSik,
Shin ChangHo,
Lee HyoJeong,
Kim Seung
Publication year - 2015
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21726
Subject(s) - luteolin , antithrombotic , chemistry , partial thromboplastin time , fibrin , pharmacology , in vivo , thromboplastin , coagulation , flavones , thrombin , thrombus , biochemistry , medicine , platelet , biology , immunology , chromatography , microbiology and biotechnology , antioxidant , quercetin
The objectives of present study were to investigate whether luteolin affects procoagulant proteinase activity and fibrin clot formation and influences thrombosis and coagulation in Sprague–Dawle rats. Luteolin significantly inhibited the enzymatic activity of thrombin and FXa activity by 29.1% and 16.2%. Luteolin also inhibited fibrin polymer formation in turbidity and microscopic analysis using fluorescent conjugate. Coagulation assay of luteolin was found to prolong activated partial thromboplastin time and prothrombin time. Moreover, luteolin protected the development of oxidative stress induced thrombosis in the FeCl 3 ‐induced carotid arterial thrombus model. This study demonstrated that luteolin may be useful by reducing or preventing thrombotic challenge and can help us better understand the antithrombotic action of luteolin.