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PP2A (PR65) in Silver Carp: cDNA Cloning and Expression Analysis
Author(s) -
Ma Junguo,
Feng Yiyi,
Xie Wenjie,
Li Xiaoyu
Publication year - 2015
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21706
Subject(s) - protein phosphatase 2 , complementary dna , open reading frame , biology , microbiology and biotechnology , rapid amplification of cdna ends , carp , peptide sequence , protein subunit , genetics , gene , fishery , fish <actinopterygii>
The full‐length sequence of the protein phosphatase 2A subunit A (PP2A‐A) cDNA from silver carp was cloned and sequenced. Additionally, the transcription of PP2A‐A in the liver, kidney, and spleen of fish after microcystin (MC)‐exposure were also determined in this study. The results reveal that PP2A‐A cDNA is 2833 base pair (bp) long and contains an open reading frame of 1767 bp encoding a protein of 589 amino acids. Sequence analysis demonstrates that PP2A is highly conserved in fish. Furthermore, the results of quantitative real‐time PCR indicate that PP2A‐A is constitutively expressed in all examined silver carp tissues and primarily in the brain and liver. Additionally, we found that 50 or 200 μg/kg of crude MC exposure significantly downregulated PP2A‐A transcription in fish liver, kidney, and spleen, suggesting that PP2A‐A may be involved in MC toxicity in silver carp.