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Spirulan from Blue‐Green Algae Inhibits Fibrin and Blood Clots: Its Potent Antithrombotic Effects
Author(s) -
Choi JunHui,
Kim Seung,
Kim SungJun
Publication year - 2015
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21690
Subject(s) - antithrombotic , fibrin , blue green algae , chemistry , pharmacology , biochemistry , medicine , biology , immunology , cyanobacteria , bacteria , genetics
We investigated in vitro and in vivo fibrinolytic and antithrombotic activity of spirulan and analyzed its partial biochemical properties. Spirulan, a sulfated polysaccharide from the blue‐green alga Arthrospira platensis , exhibits antithrombotic potency. Spirulan showed a strong fibrin zymogram lysis band corresponding to its molecular mass. It specifically cleaved Aα and Bβ, the major chains of fibrinogen. Spirulan directly decreased the activity of thrombin and factor X activated (FXa), procoagulant proteins. In vitro assays using human fibrin and mouse blood clots showed fibrinolytic and hemolytic activities of spirulan. Spirulan (2 mg/kg) showed antithrombotic effects in the ferric chloride (FeCl 3 )‐induced carotid arterial thrombus model and collagen and epinephrine‐induced pulmonary thromboembolism mouse model. These results may be attributable to the prevention of thrombus formation and partial lysis of thrombus. Therefore, we suggest that spirulan may be a potential antithrombotic agent for thrombosis‐related diseases.