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Alantolactone Induces Apoptosis and Cell Cycle Arrest on Lung Squamous Cancer SK‐MES‐1 Cells
Author(s) -
Zhao Peng,
Pan Zhenxiang,
Luo Yungang,
Zhang Leilei,
Li Xin,
Zhang Guangxin,
Zhang Yifan,
Cui Ranji,
Sun Mei,
Zhang Xingyi
Publication year - 2015
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21685
Subject(s) - cell cycle , cyclin dependent kinase 6 , apoptosis , cancer research , cell cycle checkpoint , cyclin d1 , cancer cell , caspase , chemistry , cell growth , lung cancer , cell , biology , microbiology and biotechnology , cancer , programmed cell death , biochemistry , medicine , genetics
Alantolactone, a sesquiterpene lactone compound, has variety of pharmacological properties, including anti‐inflammatory and antineoplastic effects. In our study, alantolactone inhibited cancer cell proliferation. To explore the mechanisms underlying its antitumor action, we further examined apoptotic cells and cell cycle distribution using flow cytometry analysis. Alantolactone triggered apoptosis and induced cell cycle G1/G0 phase arrest. Furthermore, the expressions of caspases‐8, ‐9, ‐3, PARP, and Bax were significantly upregulated, while antiapoptotic factor Bcl‐2 expression was inhibited. In addition, the expressions of cyclin‐dependent kinase 4 (CDK4), CDK6, cyclin D3, and cyclin D1 were downregulated by alantolactone. Therefore, our findings indicated that alantolactone has an antiproliferative role on lung squamous cancer cells, and it may be a promising chemotherapeutic agent for squamous lung cancer SK‐MES‐1 cells.