Premium
Assessment of ER Stress and Autophagy Induced by Ionizing Radiation in Both Radiotherapy Patients and Ex Vivo Irradiated Samples
Author(s) -
Saglar Emel,
Unlu Sibel,
Babalioglu Ibrahim,
Gokce Saban Cakir,
Mergen Hatice
Publication year - 2014
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21579
Subject(s) - ex vivo , ionizing radiation , bystander effect , unfolded protein response , endoplasmic reticulum , in vivo , radiosensitivity , irradiation , chemistry , autophagy , glyceraldehyde , microbiology and biotechnology , cancer research , radiation therapy , biology , dehydrogenase , medicine , enzyme , immunology , apoptosis , biochemistry , genetics , physics , nuclear physics
Acute radiation leads to several toxic clinical states and triggers some molecular pathways. To shed light on molecular mechanisms triggered by ionizing radiation (IR), we examined the expression profiles of endoplasmic reticulum (ER) stress and autophagy‐related genes in individuals who were exposed to IR. Blood samples were collected from 50 cancer patients before radiotherapy and on the 5th, 15th, and 25th days of the treatment. Peripheral blood samples from 10 healthy volunteers were also obtained for ex vivo irradiation, divided into five and irradiated at a rate of 373 kGy/h to 0, 0.1, 0.5, 1, and 3Gy γ‐rays using a constant gamma source. GRP78, ATG5, LC3, ATF4, XBP1, and GADD153 genes were analyzed by quantitative real‐time polymerase chain reaction (QRT‐PCR) using beta 2 microglobulin (B2M) and glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) as references. In both groups, expressions of the selected genes have increased. It can be concluded that IR induces ER stress and related authophagy pathway in the peripheral lymphocyte cells proportionally by dose.