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Modulation of Gene‐Expression Profiles Associated with Sodium Arsenite‐Induced Cardiotoxicity by p‐Coumaric Acid, a Common Dietary Polyphenol
Author(s) -
Prasangalakshmi,
Rasool Mahaboobkhan
Publication year - 2014
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21550
Subject(s) - sodium arsenite , kinase , chemistry , p38 mitogen activated protein kinases , transcription factor , messenger rna , cardiotoxicity , protein kinase a , microbiology and biotechnology , biochemistry , biology , toxicity , gene , arsenic , organic chemistry
In the present study, the purpose was to investigate the effect of p‐coumaric acid on the mRNA‐expression levels of inflammatory cytokines, transcription factor, MAP kinases, and apoptotic proteins by real time reverse transcription polymerase chain reaction in the cardiac tissue of sodium arsenite exposed rats. Sodium arsenite administration (5 mg/kg/b.wt, once daily for 30 days) upregulated the mRNA‐expression levels of inflammatory cytokines (interleukin–1 beta, interleukin–6, tumor necrosis factor–alpha, and tumor growth factor–beta), transcription factor (NF‐Kb‐Rel A), protein kinases (Janus kinase and p38), caspase 3, and proapoptotic protein Bax in the cardiac tissue of rats, but the antiapoptotic protein Bcl‐2 mRNA expression was found be downregulated. However, p‐coumaric acid (75, 100 mg/kg/b. wt. oral) pretreatment daily before the sodium arsenite exposure protected the changes in the above mRNA‐expression profiles observed in the cardiac tissues. In conclusion, this study confirmed that p‐coumaric acid could be a promising dietary agent for protecting against the sodium arsenite‐induced cardiotoxicity.