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Tl + Showed Negligible Interaction with Inner Membrane Sulfhydryl Groups of Rat Liver Mitochondria, but Formed Complexes with Matrix Proteins
Author(s) -
Korotkov Sergey M.,
Brailovskaya Irina V.,
Kormilitsyn Boris N.,
Furaev Viktor V.
Publication year - 2014
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21547
Subject(s) - chemistry , mitochondrion , swelling , inner membrane , sucrose , reagent , inner mitochondrial membrane , biochemistry , matrix (chemical analysis) , calcium , respiration , membrane , biophysics , chromatography , organic chemistry , anatomy , biology , chemical engineering , engineering
The effects of Tl + on protein sulfhydryl (SH) groups, swelling, and respiration of rat liver mitochondria (RLM) were studied in a medium containing TlNO 3 and sucrose, or TlNO 3 and KNO 3 as well as glutamate plus malate, or succinate plus rotenone. Detected with Ellman's reagent, an increase in the content of the SH groups was found in the inner membrane fraction, and a simultaneous decline was found in the content of the matrix‐soluble fraction for RLM, incubated and frozen in 25–75 mM TlNO 3 . This increase was greater in the medium containing KNO 3 regardless of the presence of Ca 2+ . It was eliminated completely for RLM injected in the medium containing TlNO 3 and then washed and frozen in the medium containing KNO 3 . Calcium‐loaded RLM showed increased swelling and decreased respiration. These results suggest that a ligand interaction of Tl + with protein SH groups, regardless of the presence of calcium, may underlie the mechanism of thallium toxicity.