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A Comparative Study of In Vitro Cytotoxic, Antioxidant, and Antimicrobial Activity of Pt(II), Zn(II), Cu(II), and Co(III) Complexes with N ‐heteroaromatic Schiff Base ( E )‐2‐[ N ′‐(1‐pyridin‐2‐yl‐ethylidene)hydrazino]acetate
Author(s) -
Filipović Nenad R.,
Marković Ivanka,
Mitić Dragana,
Polović Natalija,
Milčić Miloš,
Dulović Marija,
Jovanović Maja,
Savić Milena,
Nikšić Miomir,
Anđelković Katarina,
Todorović Tamara
Publication year - 2014
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21541
Subject(s) - chemistry , cytotoxic t cell , dna fragmentation , in vitro , schiff base , antimicrobial , antioxidant , stereochemistry , apoptosis , cytotoxicity , cisplatin , phosphatidylserine , lipid peroxidation , medicinal chemistry , biochemistry , membrane , programmed cell death , organic chemistry , biology , phospholipid , chemotherapy , genetics
In search for novel biologically active metal based compounds, an evaluation of in vitro cytotoxic, antioxidant, and antimicrobial activity of new Pt(II) complex and its Zn(II), Cu(II), and Co(III) analogues, with NNO tridentately coordinated N ‐heteroaromatic Schiff base ligand ( E )‐2‐[ N ′‐(1‐pyridin‐2‐yl‐ethylidene)hydrazino]acetate, was performed. Investigation of antioxidative properties showed that all of the compounds have strong radical scavenging potencies. The Zn(II) complex showed potent inhibition of DNA cleavage by hydroxyl radical. A cytotoxic action of investigated compounds was evaluated on cultures of human promyelocitic leukaemia (HL‐60), human glioma (U251), rat glioma (C6), and mouse melanoma (B16) cell lines. It was shown that binuclear pentacoordinated Zn(II) complex possesses a strong dose‐dependent cytotoxic activity, of the same order of magnitude as cisplatin on B16, C6, and U251 cells. Furthermore, Zn(II) complex causes oxidative stress‐induced apoptotic death of HL‐60 leukemic cells, associated with caspase activation, phosphatidylserine externalization, and DNA fragmentation.