Premium
Thiobencarb Herbicide Reduces Growth, Photosynthetic Activity, and Amount of Rieske Iron‐Sulfur Protein in the Diatom Thalassiosira pseudonana
Author(s) -
Shimasaki Yohei,
Tsuyama Michito,
Tasmin Rumana,
Qiu Xuchun,
Shimizu Motoyuki,
Sato Yusuke,
Yamasaki Yasuhiro,
KatoUnoki Yoko,
Nukata Atsushi,
Nakashima Takashi,
Ichinose Hirofumi,
Wariishi Hiroyuki,
Honjo Tsuneo,
Oshima Yuji
Publication year - 2013
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21505
Subject(s) - thalassiosira pseudonana , photosynthesis , electron transport chain , photosystem ii , chemistry , diatom , photosystem , photosystem i , sulfur , biophysics , botany , biochemistry , biology , organic chemistry , phytoplankton , nutrient
We investigated the effects of the herbicide thiobencarb on the growth, photosynthetic activity, and expression profile of photosynthesis‐related proteins in the marine diatom Thalassiosira pseudonana . Growth rate was suppressed by 50% at a thiobencarb concentration of 1.26 mg/L. Growth and photosystem II activity ( F v / F m ratio) were drastically decreased at 5 mg/L, at which the expression levels of 13 proteins increased significantly and those of 11 proteins decreased significantly. Among these proteins, the level of the Rieske iron‐sulfur protein was decreased to less than half of the control level. This protein is an essential component of the cytochrome b 6 f complex in the photosynthetic electron transport chain. Although the mechanism by which thiobencarb decreased the Rieske iron‐sulfur protein level is not clear, these results suggest that growth was inhibited by interruption of the photosynthetic electron transport chain by thiobencarb. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:437‐444, 2013; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.21505