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Responses of the Antioxidant System in QGY‐7701 Cells to the Cytotoxicity and Apoptosis Induced by 1‐Octyl‐3‐methylimidazolium Chloride
Author(s) -
Jing Changqin,
Li Xiaoyu,
Zhang Jihong,
Wang Jianji
Publication year - 2013
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21495
Subject(s) - chemistry , reactive oxygen species , superoxide dismutase , cytotoxicity , malondialdehyde , oxidative stress , glutathione , apoptosis , catalase , antioxidant , medicinal chemistry , biochemistry , enzyme , in vitro
This study aims to evaluate the cytotoxicity and responses of the cellular antioxidant system of 1‐octyl‐3‐methylimidazolium chloride ([C 8 mim][Cl]) on human hepatocarcinoma QGY‐7701 cells. The results show that [C 8 mim][Cl] can inhibit QGY‐7701 cell growth and decrease their viabilities in a dose‐dependent manner. The results also reveal that [C 8 mim][Cl] exposure can induce apoptosis in the [C 8 mim][Cl]‐treated QGY‐7701 cells. In addition, the results of biochemical assays show that [C 8 mim][Cl] exposure causes overproduction of reactive oxygen species (ROS), inhibits superoxide dismutase (SOD) and catalase (CAT) activities, decreases reduced glutathione (GSH) content, and increases the cellular malondialdehyde (MDA) level. These results suggest that ROS‐mediated oxidative stress may be responsible for the apoptosis induced by [C 8 mim][Cl] in QGY‐7701 cells. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:330‐336, 2013; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.21495