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Ebselen Reduces the Toxicity of Mechlorethamine in A‐431 Cells via Inhibition of Apoptosis
Author(s) -
Lulla Anju,
Pino Maria A.,
PiętkaOttlik Magdalena,
Młochowski Jacek,
Sparavalo Oleksiy,
Billack Blase
Publication year - 2013
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21490
Subject(s) - ebselen , chemistry , toxicity , nitrogen mustard , pharmacology , in vitro , in vivo , acute toxicity , biochemistry , antioxidant , superoxide dismutase , cyclophosphamide , medicine , organic chemistry , biology , chemotherapy , glutathione peroxidase , microbiology and biotechnology
A series of test compounds were evaluated for an ability to reduce the toxicity of the nitrogen mustard mechlorethamine (HN2) in vitro. The test compounds included resveratrol, pterostilbene, vitamin C, ebselen, ebselen diselenide, and ebselen‐sulfur. Among them, ebselen demonstrated the highest degree of protection against HN2 toxicity. To this end, pretreatment of the cells with ebselen offered protection against the toxicant whereas no protection was observed when cells were first incubated with HN2 and then treated with ebselen. Significant increases in caspase 3 and caspase 9 activities were observed in response to HN2, and ebselen was found to reduce these effects. Taken together, the data presented here indicate that ebselen is an effective countermeasure to nitrogen mustard in vitro, which is worthy of future investigation in vivo. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:313‐322, 2013; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.21490