Premium
Acute Effects of Pyrethroids on Serotonin Release in the Striatum of Awake Rats: An In Vivo Microdialysis Study
Author(s) -
Hossain Muhammad M.,
Suzuki Tadahiko,
Richardson Jason R.,
Kobayashi Haruo
Publication year - 2013
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21450
Subject(s) - microdialysis , deltamethrin , chemistry , striatum , serotonergic , pharmacology , serotonin , antagonist , neurotransmitter , nimodipine , tetrodotoxin , extracellular , medicine , dopamine , biology , biochemistry , calcium , receptor , organic chemistry , pesticide , agronomy
The present study examined the acute neurotoxic effects of three different pyrethroids, allethrin, cyhalothrin, and deltamethrin on the release of serotonin (5‐HT) and its metabolite 5‐hydroxyindoleacetic acid (5‐HIAA) in the striatum of conscious rats using microdialysis. Allethrin 10 mg/kg reduced extracellular levels of 5‐HT to 46%, whereas 20 and 60 mg/kg increased the release to 177% and 243% of baseline, respectively. Cyhalothrin increased 5‐HT release to 145–204% and deltamethrin decreased to 58–32% of baseline in a dose‐dependent manner. None of the pyrethroids tested altered extracellular levels of 5‐HIAA. Local infusion of the voltage‐gated sodium channel antagonist tetrodotoxin (TTX) into striatum completely prevented the effects of allethrin, cyhalothrin, and deltamethrin (10 and 20 mg/kg) on 5‐HT release. The effect of deltamethrin at 60 mg/kg was completely abolished by striatal infusion of nimodipine (L‐type Ca ++ channel antagonist) with TTX. These findings suggest that pyrethroids disrupt the serotonergic neurotransmission in striatum in a dose‐related manner with Na + and Ca 2+ channel‐dependent mechanisms. © 2012 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:150‐156, 2013; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.21450