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Proteomic analysis of mitochondria from infantile hemangioma endothelial cells treated with sodium morrhuate and its liposomal formulation
Author(s) -
Tu Junbo,
Dong Qiang,
Hu Xiaoyi,
Jiang Fei,
Ma Ruizhao,
He Linying,
Yang Zhuangqun
Publication year - 2012
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21436
Subject(s) - apoptosis , hemangioma , mitochondrion , downregulation and upregulation , liposome , lamin , cytochrome c , microbiology and biotechnology , cancer research , medicine , pathology , chemistry , biology , gene , biochemistry
Hemangioma is the most common benign tumor of infancy. The aim of this study is to evaluate the biological effects of sodium morrhuate (SM) and its liposomal formulation on infantile hemangioma endothelial cells (IHECs). Morphological analysis revealed that exposure to liposomal sodium morrhuate (LSM) preferentially caused apoptotic death in IHECs, manifested as shrunken configuration and formation of apoptotic bodies. In contrast, necrotic death was prominent in IHECs treated with an equal concentration of SM. By means of proteomic analysis and confirmation experiments, we revealed that the apoptosis‐inducing effects of LSM were associated with an upregulation of a set of genes involved in mitochondrial death pathway, including apoptosis‐inducing factor, cytochrome c1, caspase‐8, and lamin B1. In conclusion, our data highlight the proapoptotic activity of LSM in IHECs through the mitochondrial apoptotic pathway and may provide a promising avenue to treat hemangiomas of infancy. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:374–380, 2012; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.21436