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Cardamonin protects septic mice from acute lung injury by preventing endothelial barrier dysfunction
Author(s) -
Wei Zhifeng,
Yang Jian,
Xia YuFeng,
Huang WenZhe,
Wang ZhengTao,
Dai Yue
Publication year - 2012
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21420
Subject(s) - lung , medicine , endothelial dysfunction , chemistry , pharmacology , immunology , microbiology and biotechnology , biology
Cardamonin, a flavone compound isolated from Alpinia katsumadai Heyata seeds, has been reported to possess anti‐inflammatory and anticoagulative activities, and it might be beneficial for management of sepsis. This study was conducted to examine the protective effects of cardamonin on experimental sepsis and resultant acute lung injury (ALI). Cardamonin (30 and 100 mg/kg) significantly elevated the survival rate of septic mice, alleviated ALI and lung microvascular leak, and lowered the serum levels of proinflammatory cytokines TNF‐α, IL‐1β, and IL‐6. In vitro, it (25 and 50 µM) concentration dependently inhibited endothelium permeability and downregulated phosphorylation of P38 in rat lung microvascular endothelial cells induced by lipopolysaccharide (LPS). P38 inhibitor inhibited the endothelium permeability. In RAW 264.7 macrophage cells, cardamonin also showed selective inhibition of P38 phosphorylation induced by LPS. These results indicate that cardamonin can protect septic mice from ALI by preventing endothelium barrier dysfunction via selectively inhibiting P38 activation. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:282–290, 2012; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.21420