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Aloe‐emodin inhibits adipocyte differentiation and maturation during in vitro human mesenchymal stem cell adipogenesis
Author(s) -
SubashBabu Pandurangan,
Alshatwi Ali A.
Publication year - 2012
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21415
Subject(s) - adipocyte , adipogenesis , mesenchymal stem cell , chemistry , cellular differentiation , adiponectin , endocrinology , medicine , oil red o , adipose triglyceride lipase , ibmx , adipose tissue , microbiology and biotechnology , biology , biochemistry , in vitro , insulin , insulin resistance , forskolin , lipolysis , gene
In this study, we examined the effects of Aloe‐emodin (AE) on the inhibition of adipocyte differentiation during 3‐isobutyl‐1‐methylxanthine (IBMX)‐induced adipocyte differentiation in human mesenchymal stem cells (hMSCs). AE treatment (5, 10, and 20 µM) of preadipocyte cells resulted in a significant ( p < 0.05) decrease in glycerol phosphate dehydrogenase and triglyceride levels as well as an increase in lactate dehydrogenase activity and attenuated lipid accumulation compared with untreated differentiated adipocytes. Using quantitative reverse transcription polymerase chain reaction, we studied the mRNA expression levels of resistin, adiponectin, aP 2 , lipoprotein lipase, PPARγ, and tumor necrosis factor‐α in hMSCs undergoing adipocyte differentiation; treatment with AE decreased the expression of these adipogenic genes and decreased adipocyte differentiation. In addition, AE suppresses the differentiation of hMSCs into adipocytes by downregulating PPARγ and C/EBPα expressions. AE significantly inhibited hMSCs proliferation and preadipocyte differentiation within the first 2 days of treatment, indicating that the antiadipogenic effect. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:291–300, 2012; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.21415