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Iron overload potentiates diet‐induced hypercholesterolemia and reduces liver ppar‐α expression in hamsters
Author(s) -
Bonomo Larissa de Freitas,
Silva Maísa,
Oliveira Riva de Paula,
Silva Marcelo Eustáquio,
Pedrosa Maria Lucia
Publication year - 2012
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21410
Subject(s) - endocrinology , medicine , cholesterol , peroxisome proliferator activated receptor , reductase , coenzyme a , peroxisome , receptor , chemistry , biology , biochemistry , enzyme
Iron stores and lipids are related to the development of cardiovascular disease. Given that peroxisome proliferator‐activated receptor alpha (PPAR‐α) regulates important physiological processes that impact lipid and glucose homeostasis, we decided to investigate the effects of iron overload on serum lipids and the liver expression of PPAR‐α, 3‐hydroxy‐3‐methylglutaryl coenzyme A reductase, and cholesterol 7α‐hydroxylase. Hamsters were divided into four groups. The standard group (S) was fed the AIN‐93M diet, the SI group was fed the diet and iron injections, the hypercholesterolemic group (H) was fed a standard diet containing cholesterol, and the HI group was fed a high‐cholesterol diet and iron injections. Serum cholesterol in the HI group was higher than in the H group. Gene expression analysis of PPAR‐α showed that the HI group had a lower PPAR‐α expression than H. These data show that iron, when associated with a high‐fat diet, can cause increased serum cholesterol levels, possibly due to a reduction in PPAR‐α expression. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:224–229, 2012; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.21410