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Cadmium‐induced hepatotoxicity and its abrogation by thymoquinone
Author(s) -
Zafeer Mohammad Faraz,
Waseem Mohammad,
Chaudhary Shaista,
Parvez Suhel
Publication year - 2012
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.21402
Subject(s) - thymoquinone , chemistry , antioxidant , glutathione , oxidative stress , cadmium , nigella sativa , pharmacology , enzyme , biochemistry , traditional medicine , biology , medicine , organic chemistry
Cadmium (Cd 2+ ) causes alteration of the cellular homeostasis and oxidative damage. The aim of the present study was to investigate the possible protective role of thymoquinone (TQ), a predominant bioactive component present in black seed oil ( Nigella sativa ) on the hepatotoxicity of Cd 2+ with special reference to its protection against perturbation of nonenzymatic and enzymatic antioxidants. The effect of TQ pretreatment was examined in postnuclear supernatant prepared from liver of Swiss albino mice under in vitro conditions. CdCl 2 treatment (5 mM) resulted in a significant increase in antioxidant enzymatic activities. It also caused a significant ( p < 0.001) increase in protein carbonyl and reduced glutathione content. Pretreatment with TQ (10 μM) showed a significant protection as manifested by noticed attenuation of protein oxidation and rejuvenation of the depleted antioxidants of cellular fraction. These results strengthen the hypothesis that TQ exerts modulatory influence on the antioxidant defense system on being subjected to toxic insult. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:199–205, 2012; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.21402