Premium
Assessment on pollution of ochratoxin a in grain in China and its apoptosis effect on vitro‐cultured human tubular kidney cells
Author(s) -
Li Zengning,
Zhang Xianghong,
Cui Jinfeng,
Kang Weijun
Publication year - 2012
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.20420
Subject(s) - apoptosis , ochratoxin a , flow cytometry , western blot , microbiology and biotechnology , chemistry , caspase 3 , biology , programmed cell death , mycotoxin , biochemistry , food science , gene
Ochratoxin A (OTA) is nephrotoxic, immunosuppressive, and teratogenic in many species and is a possible human carcinogen. In this study, we investigated the OTA pollution situations of grains in northern China and the signaling pathway that mediated OTA‐induced apoptosis in human tubular kidney cells (HKCs). Samples of grains collected from three representative areas were determined by using high‐performance liquid chromatography fluorescence method. The effects of OTA on cell apoptosis, caspase‐3, Bax, and Bcl‐2 expression, and phosphorylation of c‐Jun NH 2 terminal kinase (JNK) were detected in cultured HKCs via flow cytometry (FCM), Hoechst 33258 staining, and Western blot. It showed that OTA pollution of edible grains was very common in north China. OTA could affect caspase‐3, Bax, and Bcl‐2 expression and increased cell apoptosis in cultured HKCs. The JNK signalling pathway might play an important role during these cellular events. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:139–146, 2012; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.20420