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Metabolic adaptations to arsenic‐induced oxidative stress in male wistar rats
Author(s) -
Rana Tanmoy,
Bera Asit Kumar,
Das Subhashree,
Bhattacharya Debasis,
Pan Diganta,
Das Subrata Kumar
Publication year - 2012
Publication title -
journal of biochemical and molecular toxicology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.526
H-Index - 58
eISSN - 1099-0461
pISSN - 1095-6670
DOI - 10.1002/jbt.20416
Subject(s) - arsenic , sod2 , oxidative stress , arsenite , superoxide dismutase , arsenic toxicity , endocrinology , glyceraldehyde 3 phosphate dehydrogenase , chemistry , medicine , arsenic poisoning , physiology , biology , biochemistry , dehydrogenase , enzyme , organic chemistry
The present study was planned to investigate the effect of arsenic in rats on several biochemical indices of oxidative stress. Rats were exposed to arsenite in drinking water for upto 12 weeks. Chronic exposure to arsenic for a period of 12 weeks significantly ( p < 0.05) increased arsenic burden in blood, liver, and kidney. Several intrinsic antioxidant defenses were activated after a 4‐week exposure to arsenic. Some remained elevated, but others became depressed over a longer exposure period. Alterations in most of the biochemical variables reached statistical significant ( p < 0.05). Arsenic significantly ( p < 0.01) reduced mRNA expression of the superoxide dismutase 2 (SOD2) gene with respect to the glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) gene. These observations indicated that prolong exposure to arsenic causes induction of oxidative stress and biochemical alterations. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:101–116, 2012; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.20416